ASI and Special Optics have developed two dipping objective lenses designed for light sheet microscopy of cleared tissue samples, including ASI’s ct-dSPIM. These objectives work in any refractive index media without a correction collar because of a unique curved first surface. They are robust to immersion in harsh media including DBE and BABB.
The original objective lens (54-10-12) has nominal NA of 0.4, WD of 12 mm, and allows imaging over 5 mm deep into a flat sample. The second one (54-12-8) has an increased NA of 0.7 with a WD of 10 mm. Both NA and magnification vary slightly with the refractive index of the immersion medium, but WD is constant.
Cleared Tissue Objective Features
- Dual-view cleared tissue imaging (diSPIM with cleared tissues)
- Single-view cleared tissue imaging (OpenSPIM setups for cleared tissues)
- Water-dipping applications with very long working distances