Custom Objective Lenses Support Two-Photon Microscopy Applications
Special Optics offers objective lens solutions optimized for use in two-photon microscopy systems.These apochromatic objectives are customized with high numerical apertures and long working distances for use with various sample sizes and applications. Biomedical researchers use two-photon microscopy to image tissue and look deeper into samples to determine their nature and structure.
Two-photon microscopy is a fluorescence imaging technique used to detail tissue up to a depth of one millimeter. This technique is widely utilized in biotechnology and life sciences, and uses red-shifted light to excite fluorophores. A high-energy pulsed laser delivers bursts of high-frequency illumination. For each excitation, two photons of infrared light are absorbed; using infrared light minimizes scattering in the sample tissue, and allows for deeper tissue penetration and reduced phototoxicity. Layers of fluorescently labeled samples are gradually built up, creating three-dimensional images of living tissue, such as neural and embryonic cells.
Special Optics' custom objectives meet the need for working distances from 0.3 mm to a longerrange of 30 mm. Lenses are also tailored to function at wavelengths from visible light (390-750 nm) to the near infrared (700-1400 nm). Depending on the type of studies being done, two-photon microscopy systems may also be modified for aqueous, oil, and vacuum research environments.
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